Optimization of Spray Drying Conditions for Yield, Particle Size and Biological Activity of Thermally Stable Viral Vectors.Purpose. This work examines the relevance of viral activity in the optimization of spray drying process parameters for the development of thermally stable vaccine powders.In some instances, the actual active pharmaceutical ingredient API is not included in the process optimization as it is deemed too costly to use until the final selection of operating conditions, however, that approach is inappropriate for highly labile biopharmaceutics.We investigate the effects of spray drying parameters on i yield, ii particle size and iii viral vector activity of a mannitoldextran encapsulated recombinant human type 5 adenoviral vector vaccine, to demonstrate the effects and magnitude of each effect on the three responses, and further show that the API must be included earlier in the optimization.Methods. A design of experiments approach was used with response surface methodology RSM to optimize parameters including inlet temperature, spray gas flow rate, liquid feed rate and solute concentration in the feed.Results. In general, good conditions for maintaining viral activity led to reduced yield and fewer particles of the desired size.Within the range of parameters tested, the yield varied from 5.Conclusions. RSM indicates that the most significant spray drying parameters are the inlet temperature and spray gas flow rate.It was not possible to optimize all three output variables with one set of parameters, indicating that there will only be one dominant criteria for processing which in the case of viral vaccines will likely be viral vector activity.KEY WORDSadenovirus optimization particle size process parameters spray dry surface response methodology titre viral vector yield.Submissions from 2014.Amoroso, Jon William 2014 Reactive Probes for Manipulating Polyketide Synthases, and Photoreactive Probes for Strained Alkyne Click Chemistry.Doctoral Dissertations Available from Proquest Dissertations and Theses.Submissions from 2.Amoroso, Jon William 2.Reactive Probes for Manipulating Polyketide Synthases, and Photoreactive Probes for Strained Alkyne Click Chemistry Bayram, Armagan 2.Stochastic dynamic optimization models for societal resource allocation Becker, Georg Tobias 2.Investigating The Effect Of Ph On Amylase Activity Variables Math' title='Investigating The Effect Of Ph On Amylase Activity Variables Math' />Intentional and unintentional side channels in embedded systems Biddle, Amy Sanders 2.Anaerobic microbes and communities in the context of soil and the equine digestive tract Clark, Daniel E 2.Roosting, site fidelity, and food sources of urban gulls in massachusetts Implications for protecting public water supplies Colvin, Kimberly F 2.Effect of automatic item generation on ability estimates in a multistage test Conway, Leslie C 2.Biophysical studies of axonal transport Crocker, Jillian M 2.Contours of workplace resistance Organization, collaboration, and recognition De.George, Joan 2. 01.Individual differences in psychotherapy change among ethnic minority patients Della Pelle, Andrea M 2.Design and syntheses of donor acceptor dyads and triads for improved light harvesting in organic photovoltaics Dharmasiri, Kanchuka N 2.Transgressing space and subverting hierarchies a comparative analysis of street theatre groups in Sri Lanka, India, and the United States Dong, Jia 2.Mass spectrometry methods for studying protein metal binding Earnest, Evan J 2.Investigating the role of hydromechanical coupling in shallow, fractured rock aquifers Ercan, Ilke 2.Heat dissipation bounds for nanocomputing Methodology and applications Feild, Jacqueline L 2.Improving text recognition in images of natural scenes Fielding, Peter G 2.The traditional vocal repertoire of nova scotia A classification of pitch space Gummeson, Jeremy 2.Exploiting energy harvesting for passive embedded computing systems Gu, Xiaodan 2. 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